| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Clostridium butyricum | GTGGCGTACAATGAATTTGCA | TGCAACATTACCTGTCCCACA | 58.06 | 59.86 | 156 | 127 |
100.00%
|
50.00%
|
| Primer 42 | Clostridium butyricum | GTGGCGTACAATGAATTTGCA | GCAACATTACCTGTCCCACA | 58.06 | 58.10 | 155 | 127 |
100.00%
|
50.00%
|
| Primer 43 | Clostridium butyricum | TGGAGCAAACGGTGTTGGA | ACGTAGCCCAAAAGCCACA | 59.78 | 59.85 | 208 | 127 |
100.00%
|
50.00%
|
| Primer 44 | Clostridium butyricum | AAGACTTGGGCTTGGACCG | TGGAATCCACATGACACCACT | 59.93 | 59.30 | 276 | 125 |
100.00%
|
50.00%
|
| Primer 45 | Clostridium butyricum | GTGGCGTACAATGAATTTGCA | ACTGCAACATTACCTGTCCCA | 58.06 | 59.58 | 158 | 125 |
100.00%
|
50.00%
|
| Primer 46 | Clostridium butyricum | TCTCCAGGAGTAAAGCTTGGA | AACTACCGCACCAGCTCCA | 58.10 | 61.21 | 261 | 125 |
100.00%
|
50.00%
|
| Primer 47 | Clostridium butyricum | ACAGTGGCTGCATCAGGTA | CGCTATTACATGGGCAACAGC | 58.62 | 60.00 | 171 | 123 |
100.00%
|
50.00%
|
| Primer 48 | Clostridium butyricum | AGAAATGAACGCAAGCGGAG | TCGTCGTGACCCTTGTAAGT | 59.20 | 58.68 | 195 | 123 |
100.00%
|
50.00%
|
| Primer 49 | Clostridium butyricum | TGGGAAAGAAAGGGACGTGG | CTGCTCCGCATGCAACTTC | 59.89 | 59.86 | 154 | 123 |
100.00%
|
50.00%
|
| Primer 50 | Clostridium butyricum | TGGGAAAGAAAGGGACGTGG | TGCTCCGCATGCAACTTC | 59.89 | 58.73 | 153 | 123 |
100.00%
|
50.00%
|